Indexed by:
期刊论文
First Author:
Wang, Lin
Correspondence Author:
Jiang, XB (reprint author), Dalian Univ Technol, Engn Lab Petrochem Energy Efficient Separat Techn, Sch Chem Engn, State Key Lab Fine Chem, Dalian 116024, Liaoning, Peoples R China.
Co-author:
He, Gaohong,Ruan, Xuehua,Zhang, Daishuang,Xiao, Wu,Li, Xiangcun,Wu, Xuemei,Jiang, Xiaobin
Date of Publication:
2018-08-08
Journal:
ACS APPLIED MATERIALS & INTERFACES
Included Journals:
PubMed、SCIE
Document Type:
J
Volume:
10
Issue:
31
Page Number:
26653-26661
ISSN No.:
1944-8244
Key Words:
hydrogel composite membrane; continuous protein crystallization;
morphology selectivity; nucleation and growth control; lysozyme
Abstract:
The tailored and robust hydrogel composite membranes (HCMs) with diverse ion adsorption and interfacial nucleation property are prepared and successfully used in the continuous lysozyme crystallization. Beyond the heterogeneous supporter, the HCMs functioning as an interface ion concentration controller and nucleation generator are demonstrated. By constructing accurately controlled nucleation and growth circumstances in the HCM-equipped membrane crystallizer, the target desired morphology (hexagon cube) and brand-new morphology (multiple flower shape) that differ from the ones created in the conventional crystallizer are continuously and repetitively generated with ultrahigh morphology selectivity. These tailored robust HCMs show great potential for improving current approaches to continuous protein crystallization with specific crystal targets from laboratorial research to actual engineering applications.
Translation or Not:
no
