Indexed by:
期刊论文
First Author:
Wang, Ziqian
Correspondence Author:
Zhang, ZC (reprint author), Dalian Univ Technol, Sch Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China.
Co-author:
Xu, Wenjie,Song, Ting,Guo, Zongwei,Liu, Lu,Fan, Yudan,Wang, Anhui,Zhang, Zhichao
Date of Publication:
2017-01-01
Journal:
ARCHIV DER PHARMAZIE
Included Journals:
SCIE、PubMed、Scopus
Document Type:
J
Volume:
350
Issue:
1
ISSN No.:
0365-6233
Key Words:
Fragment-based drug design; Ligand efficiency; Mcl-1 inhibitor; p1
pocket; Selective
Abstract:
Based on a known selective Mcl-1 inhibitor, 6-chloro-3-(3-(4-chloro-3,5-dimethylphenoxy)propyl)-1H-indole-2-carboxylic acid, we applied a fragment-based approach to obtain new molecules that extended into the p1 pocket of the BH3 groove and then exhibited binding selectivity for the Mcl-1 over the Bcl-2 protein. After we deconstructed the 1H-indole-2-carboxylic acid from the parental molecule, a benzenesulfonyl was substituted at the 1-position to adopt a geometry preferred for accessing the p1 pocket according to the binding mode of the parental molecule identified by X-ray crystallography. A linear relationship between the free energy of ligand binding (Delta G) and the count of non-hydrogen heavy atoms (HAC) was maintained during the molecular growing to occupy the p1 pocket. Finally, we not only obtained compound 12 with a 7.5-fold selectivity to Mcl-1 (K-i = 0.48 mu M by fluorescence polarization) over Bcl-2 (K-i = 3.6 mu M), but also provided evidence that additional occupation of the p1 pocket is more favorable for Mcl-1 than for Bcl-2 binding, and contributes more to Mcl-1 inhibition than occupation of the p2 pocket. Compound 12 exhibited a selective killing ability on Mcl-1-dependent cancer cells.
Translation or Not:
no
